Home Page
About SAEN
Articles and Reports
Contact Us
Events and Campaigns
Fact Sheets
Financial Information
How You Can Help
Make a Donation, Please!
Media Coverage
Newsletters
Petitions
Picture Archive
Press Releases
Resources and Links
Grass Roots Org. List
|
Stop Animal
Exploitation NOW!
S. A. E. N.
"Exposing the truth to wipe
out animal experimentation"
Resources and Links
Facility Reports and Information
Brooks Air Force Base, TX
Protocol - 2003-07 - Development of a Chronic Wound Eschar Model in Swine
Title: |
Development of a Chronic Wound Eschar Model in Swine for the
Evaluation of Debridement Modalities |
Research Category:
|
M6: Combat Casualty Care |
FY:
2003
Funding (in dollars): |
100,000 |
Responsible Organization: |
AIR FORCE RESEARCH LAB/BROOKS
|
Primary Contact: |
Public Affairs Office |
City: |
Brooks City-Base |
State: |
TX
|
Zip: |
78235-5116 |
Performing Organization: |
See Responsible Organization Information |
City: |
|
State: |
|
Zip: |
|
Keywords: |
LABORATORY ANIMALS PORCINE ESCHAR FORMATION WOUNDS DEBRIDEMENT
ENZYMATIC DEBRIDEMENT AGENT CASUALTY WOUND CARE
|
Objective: |
The work is being performed under a Cooperative Research and
Development Agreement (CRADA) with a commercial health care
products company (Healthpoint Ltd). There are two objectives in
this project 1. To produce a reproducible model of necrotic wounds
at Brooks City Base. a. One specific for burns b. One excisional
wounds.A necrosing agent is required to induce eschar formation
(in non-thermal injuries). 2. To determine whether trichrome stain
(or Martius Yellow - Scarlett Blue stain) is effective at
determining the depth of necrotic tissue in both thermal and
excisional wounds. |
Approach: |
Several publications describe the benefits of early debridement of
wounded skin. Early debridement prevents numerous wound
complications and facilitates rapid wound repair. In many
battlefield circumstances, surgical debridement is not an option.
However, enzymatic debridement, if done effectively, may be useful
in promoting the healing process. In order to discover and
demonstrate the effectiveness of enzymatic debriding agents, it is
necessary to develop a model to test these materials in vivo. This
protocol describes the route to evaluating several different
methods to arrive at a well-defined and standardized model for
debridement studies. This project is designed to develop an animal
model of eschar debridement. Wounds will be created on the backs
of domestic Yorkshire cross swine and eschar formation will be
allowed to proceed naturally, or may be assisted by application of
dermal penetration enhancers or necrosing agents. The model, once
developed, will be used to assess the efficacy of novel debriding
agents. Model development will be divided into two separate
phases. The first phase will examine the formation of eschar
created by thermally induced wounds and wounds created by
full-thickness excision and which will receive additional
treatment with common pharmaceutical agents that are designed to
maximize skin penetration for the delivery of active agents to
deeper tissues, this penetrating agent will be used as a necrosing
agent to create a non-thermal eschar. After the completion of this
phase, models will be chosen for the thermal and non-thermal
wounding methods that appear to provide the best eschar. The
second phase will establish the reproducibility of the chosen
method from phase one of the study. |
|
Return to Brooks Air Force Base, TX
Return to Texas
Return to Facility Reports and Information
Return to Resources and Links
Rats, mice, birds, amphibians and other animals have
been excluded from coverage by the Animal Welfare Act. Therefore research
facility reports do not include these animals. As a result of this
situation, a blank report, or one with few animals listed, does not mean
that a facility has not performed experiments on non-reportable animals. A
blank form does mean that the facility in question has not used covered
animals (primates, dogs, cats, rabbits, guinea pigs, hamsters, pigs,
sheep, goats, etc.). Rats and mice alone are believed to comprise over 90%
of the animals used in experimentation. Therefore the majority of animals
used at research facilities are not even counted.
|